In vitro fertilisation (IVF) :

ivf process

In vitro fertilisation (IVF) is one of several techniques available to help people with fertility problems have a baby.

During IVF, an egg is removed from the woman’s ovaries and fertilised with sperm in a laboratory. The fertilised egg, called an embryo, is then returned to the woman’s womb to grow and develop.

It can be carried out using your eggs and your partner’s sperm, or eggs and/or sperm from donors.

 

The cycle before the treatment

Step 1: The cycle before the actual IVF treatment begins you may be put on medications to control your menstrual cycle and depending on the protocol you may be required to take injections.

Step 2: When you get your cycles, on the second day of your period, you will undergo an Ultrasound to check the ovaries and look for ovarian cysts and start the treatment.

Step 3: Ovarian stimulation will be done with gonadotropin injections and frequent monitoring will be done with ultrasound to look in to the follicular growth and development. There may be need to do blood tests to evaluate the estrogen levels and progesterone to rule out premature leutanization. Once the follicles attain the dominant size, egg retrieval will be scheduled after 36 hours of HCG trigger.

Step 4: Egg retrieval or ovum pick up is planned under general anesthesia, transvaginal USS guided oocyte retrieval is preferred, a needle is used to gently aspirate the follicle and then transferred to the Embryology lab for assessment of oocytes.After the procedure you will be monitored for few hours and then discharged, light spotting and abdominal cramps is common after ovum pick up.

Step 5: Fertilization: On the day of egg retrieval husbands semen sample will be collected, sperms from washed and processed sample will be selected for doing ICSI of the mature oocytes. The fertilization will be checked 16-18 hours after doing ICSI and the embryos allowed growing in the lab for few more days.

Step 6: Embryo transfer: The fertilized eggs now termed as the embryos are then transferred in to the uterus after 3 – 5 days using an embryo transfer catheter. The number of embryos transferred will depend on the quality of the embryos and also the age of the patient. Post the transfer you will be given rest for 3 hours and then discharged.

Blastocyst Culture

Blastocyst is an embryo that has developed for five days after fertilization. Most of Embryo transfers are performed on day 2 or day 3. Recently, improvement in embryo culture condition has allowed sustained embryo development to the blastocyst stage. It is stage at which embryo naturally arrives to the uterine cavity and implantation occurs. Preliminary studies with new media and age in good responder woman have shown that ~50% of the fertilized eggs reach blastocyst stage.

Advantages of blastocyst culture :-
Better selection of the embryos to be transferred
Better selection of the embryos to be transferred
Limit the number of embryos to be transferred
Eliminate the risk of multiple pregnancy
Better implantation rate

Disadvantages of Blastocyst culture:-
Longer time interval between retrieval and transfer
No embryos or fewer embryos for freezing
Risk of embryo arrest

Candidate for blastocyst transfer:-
Woman with at least six number of good quality embryos on day II after oocyte pick up
Woman under age 35
Oocyte recipients who receives young oocytes from donor

Step 7: Luteal support: This is started soon after your egg retrieval is done, progesterone injections are given daily and estrogen supplements started to prepare the uterus to receive the pregnancy. Post embryo transfer also this support is continued till the pregnancy test is done.

Step 8: Pregnancy test and follow up: After 15 days of embryo transfer, pregnancy test is done, this will usually be a blood test, if the test is positive the progesterone support is continued for few more weeks.

First , you take medications to make multiple follicles begin to develop on your ovaries. This step is referred to as ovarian stimulation, or super ovulation.

Step two involves monitoring follicular growth by ultrasound, to determine egg growth and uterine lining development. When it is determined that the follicles and the uterine lining are appropriately mature, a trigger shot of Human Chorionic Gonadotropin is then administered.

36 hours after the trigger shot, the Third step begins with retrieval of the The success rates of our Center are amongst the best in the country.

 

 

IVF is basically a four step process.

eggs by ultrasound-guided-needle aspiration, an in-office procedure. A sperm specimen is then washed and prepared for insemination. The washed sperm is then placed in a dish with the eggs, and they are placed in an incubator for 18 hours. After 18 hours, the embryos are observed for normal fertilization, under a microscope, where the pronucleus of egg and sperm can be seen. The embryos are then incubated for further development into multi-cell embryos.

The fourth and final step involves transferring the embryos into the uterine cavity via a catheter inserted through the cervix. The number returned varies with the desires of the patient, under the guidelines of age categories; under 35 years old, up to three embryos; 35-40 years and older, up to four embryos. Additional embryos may be frozen and stored for future use.

Our goal is nothing short of perfection. We understand you have placed your future family in our hands… you won’t be disappointed. Please contact us for more information